Simplified method for the rubella haemagglutination inhibition screening test.

نویسندگان

  • P P Mortimer
  • S M Jordan
  • I V Smith
چکیده

A modification of the rubella haemagglutination inhibition (HAI) test in which both pretreatment and serum titration are carried out in wells of the same microtitre plate saves time, labour, and materials and gives results comparable to a conventional HAI procedure.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Comparison of radial haemolysis with haemagglutination inhibition in estimating rubella antibody.

Radial haemolysis (RH) for assaying rubella antibody was studied in parallel tests with haemagglutination-inhibition (HAI) on 1102 sera from adult women. Simple to perform and economical in reagents, the RH test is as sensitive as the HAI test and is a useful method of screening antenatal and other sera for rubella immunity. It detects IgG but not IgM antibody.

متن کامل

Study of discrepancies in rubella haemagglutinin titrations and a reappraisal of diluents used in the rubella haemagglutination inhibition technique.

To elucidate inconsistencies in rubella haemagglutinin assays the components of the assay technique were examined. The results of carefully controlled assays of rubella haemagglutinin antigens from different sources in various plates and diluents with four species of indicator cells are reported. The quality and quantity of gelatin in the dextrose-gelatin-veronal buffered diluent commonly used ...

متن کامل

Comparison of passive haemagglutination and haemagglutination-inhibition techniques for detection of antibodies to rubella virus.

Because of the technical problems and length of time involved in the satisfactory performance of the haemagglutination-inhibition test for detection of rubella-specific antibodies, a commercially available passive haemagglutination (PHA) kit utilising rubella antigen-sensitised human erythrocytes was tested for its suitability for use in diagnostic laboratories. The immune response to acute rub...

متن کامل

Single-sample diagnosis of recent rubella by fractionation of antibody on Sephadex G-200 column.

To facilitate the diagnosis of recent rubella infection, rubella haemagglutination inhibiting antibody has been determined in four fractions obtained by Sephadex G-200 gel filtration of samples of serum. All the 21 samples collected at the convalescent stage of the disease had varying proportions of haemagglutination inhibiting antibody in fraction 1, representing the major portion of IgM antib...

متن کامل

Detection of rubella haemagglutination-inhibition (HAI) and virus-specific IgM antibody using trypsin-treated human group O erythrocytes in the HAI test.

The modification of the standard rubella haemagglutination-inhibition (HAI) test using trypsin-treated human group O erythrocytes instead of chick erythrocytes was evaluated. In a comparative study we found that, of 816 samples tested by both methods, the titres of 807 (98.9%) sera were in close agreement within an acceptable twofold difference. Trypsin-treated human group O erythrocytes usuall...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical pathology

دوره 30 7  شماره 

صفحات  -

تاریخ انتشار 1977